Feasibility, Long-term Safety and Immune Monitoring of Regulatory T Cell Therapy in Living Donor Kidney Transplant Recipients

Short-term outcomes in kidney transplantation are marred by progressive transplant failure and mortality secondary to immunosuppression toxicity. Immune modulation with autologous polyclonal regulatory T cell (Treg) remedy might facilitate immunosuppression discount selling higher long-term scientific outcomes.

In a Phase I scientific trial, 12 kidney transplant recipients acquired 1-10×10⁶ Treg per kg at Day+5 put up transplantation in lieu of induction immunosuppression (Treg Therapy cohort). Nineteen sufferers acquired customary immunosuppression (Reference cohort). Primary outcomes have been rejection-free and affected person survival. Patient and Transplant survival was 100%; acute rejection-free survival was 100% in the Treg Therapy vs 78.9% in the reference cohort at 48 months post-transplant.

Treg remedy revealed no extra security issues. Four sufferers in the Treg Therapy cohort had mycophenolate mofetil withdrawn efficiently and stay on tacrolimus monotherapy. Treg infusion resulted in a long-lasting dose-dependent improve in peripheral blood Tregs along with a rise in marginal zone B cell numbers. We recognized a pre-transplantation immune phenotype suggesting a excessive threat of unsuccessful ex-vivo Treg enlargement.

Autologous Treg remedy is possible, protected and is probably related to a decrease rejection price than customary immunosuppression. Treg remedy might present an thrilling alternative to attenuate immunosuppression remedy and enhance long-term outcomes.

Immune thrombocytopenia (ITP) World Impact Survey (I-WISh): affected person and doctor perceptions of prognosis, indicators and signs, and remedy

Immune thrombocytopenia (ITP) is now well-known to cut back sufferers’ health-related-quality-of-life. However, knowledge describing which indicators and signs sufferers and physicians understand as having the best impression are restricted, as is knowing the total results of ITP therapies. I-WISh (ITP World Impact Survey) was an exploratory, cross-sectional survey designed to determine the multifaceted impression of ITP, and its therapies, on sufferers’ lives.

It centered on perceptions of 1507 sufferers and 472 physicians from 13 international locations concerning diagnostic pathway, frequency and severity of indicators and signs, and remedy use. Twenty-two p.c of sufferers skilled delayed prognosis (attributable to a number of components), 73% of whom felt anxious in consequence.

Patients rated fatigue among the many most frequent, extreme symptom related to ITP at prognosis (58% most frequent; 73% most extreme), though physicians assigned it decrease precedence (30%). Fatigue was one of the few signs persisting at survey completion (50% and 65%, respectively) and was the highest symptom sufferers wished resolved (46%). Participating physicians have been skilled at treating ITP, thereby recognizing the necessity to restrict corticosteroid use to newly-diagnosed or first-relapse sufferers and espoused elevated use of thrombopoietin receptor agonists and anti-CD20 after relapse in sufferers with persistent/continual illness.

Patient and physicians have been largely aligned on prognosis, signs, and remedy use. I-WISh demonstrated that sufferers and physicians largely align on total ITP symptom burden, with sure variations eg fatigue. Understanding the emotional and scientific toll of ITP on the affected person will facilitate shared decision-management, setting and institution of remedy targets and illness stage-appropriate remedy choice.

Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells

Human intestinal enteroids derived from grownup stem cells supply a related ex vivo system to review organic processes of the human intestine. They recreate mobile and practical options of the intestinal epithelium of the small gut (enteroids) or colon (colonoids) albeit restricted by the dearth of related cell sorts that assist keep tissue homeostasis and reply to exterior challenges.

In the intestine, innate immune cells work together with the epithelium, assist barrier operate, and deploy effector features. We have established a co-culture system of enteroid/colonoid monolayers and underlying macrophages and polymorphonuclear neutrophils to recapitulate the mobile framework of the human intestinal epithelial area of interest. Enteroids are generated from biopsies or resected tissue from any phase of the human intestine and maintained in long-term cultures as three-dimensional buildings by means of supplementation of stem cell progress components.

Immune cells are remoted from contemporary human complete blood or frozen peripheral blood mononuclear cells (PBMC). Monocytes from PBMC are differentiated into macrophages by cytokine stimulation previous to co-culture. The strategies are divided into the 2 primary parts of the mannequin: (1) producing enteroid/colonoid monolayers and isolating immune cells and (2) meeting of enteroid/colonoid-immune cell co-cultures with separate apical and basolateral compartments. Co-cultures containing macrophages could be maintained for 48 hr whereas these involving neutrophils, resulting from their shorter life span, stay viable for Four hr.

Enteroid-immune co-cultures allow a number of final result measures, together with transepithelial resistance, manufacturing of cytokines/chemokines, phenotypic evaluation of immune cells, tissue immunofluorescence imaging, protein or mRNA expression, antigen or microbe uptake, and different mobile features. © 2020 Wiley Periodicals LLC.

Autoimmune Regulator (AIRE) Antibody
20-abx171376	Abbexa	EUR 857.00 EUR 439.00	1 mg 200 ug

Autoimmune Regulator (AIRE) Antibody
20-abx312575	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Autoimmune Regulator (AIRE) Antibody
20-abx323992	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Autoimmune Regulator (AIRE) Antibody
abx230241-100ug	Abbexa	100 ug	EUR 481

Autoimmune Regulator (AIRE) Antibody
20-abx175527	Abbexa	EUR 1205.00 EUR 578.00	1 mg 200 ug

Autoimmune Regulator (AIRE) Antibody
20-abx175528	Abbexa	EUR 1233.00 EUR 592.00	1 mg 200 ug

Autoimmune Regulator (AIRE) Antibody
20-abx175529	Abbexa	EUR 1233.00 EUR 592.00	1 mg 200 ug

Autoimmune Regulator (AIRE) Antibody
abx331461-100ul	Abbexa	100 ul	EUR 425

Autoimmune Regulator (AIRE) Antibody
20-abx325675	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

Autoimmune regulator (AIRE) Antibody
48129-100ul	SAB	100ul	EUR 333

Autoimmune regulator (AIRE) Antibody
48129-50ul	SAB	50ul	EUR 239

Autoimmune regulator (AIRE) Antibody
48149-100ul	SAB	100ul	EUR 333

Autoimmune regulator (AIRE) Antibody
48149-50ul	SAB	50ul	EUR 239

Autoimmune Regulator (AIRE) Antibody (HRP)
20-abx312576	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Autoimmune Regulator (AIRE) Antibody (FITC)
20-abx312577	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Autoimmune Regulator (AIRE) Antibody (Biotin)
20-abx312578	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Human Autoimmune Regulator (AIRE) Protein
20-abx652629	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Autoimmune Regulator (AIRE) Protein
20-abx652630	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Mouse Autoimmune Regulator (AIRE) Protein
20-abx652631	Abbexa	EUR 578.00 EUR 258.00 EUR 1720.00 EUR 690.00 EUR 425.00	100 ug 10 ug 1 mg 200 ug 50 ug

Autoimmune regulator (AIRE) Conjugated Antibody
C48129	SAB	100ul	EUR 397

Human Autoimmune Regulator ELISA kit
E01A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Human Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Autoimmune Regulator ELISA kit
E01A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Human Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Autoimmune Regulator ELISA kit
E01A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Human Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Autoimmune Regulator ELISA kit
E08A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Autoimmune Regulator ELISA kit
E08A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Autoimmune Regulator ELISA kit
E08A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Autoimmune Regulator ELISA kit
E04A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Autoimmune Regulator ELISA kit
E04A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Autoimmune Regulator ELISA kit
E04A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Autoimmune Regulator ELISA kit
E09A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Autoimmune Regulator ELISA kit
E09A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Autoimmune Regulator ELISA kit
E09A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Autoimmune Regulator ELISA kit
E02A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Autoimmune Regulator ELISA kit
E02A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Autoimmune Regulator ELISA kit
E02A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Autoimmune Regulator ELISA kit
E07A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Porcine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Autoimmune Regulator ELISA kit
E07A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Porcine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Autoimmune Regulator ELISA kit
E07A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Porcine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Autoimmune Regulator ELISA kit
E06A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Goat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Autoimmune Regulator ELISA kit
E06A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Goat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Autoimmune Regulator ELISA kit
E06A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Goat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Autoimmune Regulator ELISA kit
E03A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Autoimmune Regulator ELISA kit
E03A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Autoimmune Regulator ELISA kit
E03A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Autoimmune Regulator (AIRE) ELISA Kit
20-abx150773	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse Autoimmune Regulator (AIRE) ELISA Kit
20-abx153694	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Human Autoimmune Regulator (AIRE) ELISA Kit
abx570732-96tests	Abbexa	96 tests	EUR 707

Mouse Autoimmune Regulator (AIRE) ELISA Kit
abx570926-96tests	Abbexa	96 tests	EUR 739

Human Autoimmune Regulator (AIRE) CLIA Kit
20-abx493615	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse Autoimmune Regulator (AIRE) CLIA Kit
20-abx493616	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Human AIRE/ Autoimmune regulator ELISA Kit
E0100Hu	Sunlong	1 Kit	EUR 605

Pig Autoimmune Regulator (AIRE) ELISA Kit
abx360670-96tests	Abbexa	96 tests	EUR 825

Rabbit Autoimmune Regulator (AIRE) ELISA Kit
abx363707-96tests	Abbexa	96 tests	EUR 825

Chicken Autoimmune Regulator (AIRE) ELISA Kit
abx355828-96tests	Abbexa	96 tests	EUR 825

Monkey Autoimmune Regulator (AIRE) ELISA Kit
abx359019-96tests	Abbexa	96 tests	EUR 825

Human Autoimmune Regulator (AIRE) CLIA Kit
abx196471-96tests	Abbexa	96 tests	EUR 825

Human Autoimmune Regulator (AIRE) ELISA Kit
abx250784-96tests	Abbexa	96 tests	EUR 707

Human Autoimmune Regulator (AIRE) ELISA Kit
DLR-AIRE-Hu-48T	DL Develop	48T	EUR 517
Description: A sandwich quantitative ELISA assay kit for detection of Human Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit
DLR-AIRE-Hu-96T	DL Develop	96T	EUR 673
Description: A sandwich quantitative ELISA assay kit for detection of Human Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit
DLR-AIRE-Mu-48T	DL Develop	48T	EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit
DLR-AIRE-Mu-96T	DL Develop	96T	EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit
RD-AIRE-Hu-48Tests	Reddot Biotech	48 Tests	EUR 521

Human Autoimmune Regulator (AIRE) ELISA Kit
RD-AIRE-Hu-96Tests	Reddot Biotech	96 Tests	EUR 723

Mouse Autoimmune Regulator (AIRE) ELISA Kit
RD-AIRE-Mu-48Tests	Reddot Biotech	48 Tests	EUR 533

Mouse Autoimmune Regulator (AIRE) ELISA Kit
RD-AIRE-Mu-96Tests	Reddot Biotech	96 Tests	EUR 740

Human Autoimmune Regulator (AIRE) ELISA Kit
RDR-AIRE-Hu-48Tests	Reddot Biotech	48 Tests	EUR 544

Human Autoimmune Regulator (AIRE) ELISA Kit
RDR-AIRE-Hu-96Tests	Reddot Biotech	96 Tests	EUR 756

Mouse Autoimmune Regulator (AIRE) ELISA Kit
RDR-AIRE-Mu-48Tests	Reddot Biotech	48 Tests	EUR 557

Mouse Autoimmune Regulator (AIRE) ELISA Kit
RDR-AIRE-Mu-96Tests	Reddot Biotech	96 Tests	EUR 774

ELISA kit for Human Autoimmune regulator
EK3200	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Autoimmune regulator in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit
SEC322Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit
SEC322Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit
SEC322Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit
SEC322Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit
4-SEC322Hu	Cloud-Clone	EUR 4782.00 EUR 2526.00 EUR 640.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Autoimmune Regulator (AIRE) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Mouse Autoimmune Regulator (AIRE) ELISA Kit
SEC322Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit
SEC322Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 488.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit
SEC322Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 654.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit
SEC322Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2644.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Autoimmune Regulator (AIRE) in Tissue homogenates and other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit
4-SEC322Mu	Cloud-Clone	EUR 4913.00 EUR 2595.00 EUR 655.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Autoimmune Regulator (AIRE) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Autoimmune Regulator(AIRE)ELISA Kit
QY-E00071	Qayee Biotechnology	96T	EUR 361

Human AIRE(Autoimmune regulator) ELISA Kit
EH1501	FN Test	96T	EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Mouse Autoimmune regulator, Aire ELISA KIT
ELI-11972m	Lifescience Market	96 Tests	EUR 865

Human Autoimmune regulator, AIRE ELISA KIT
ELI-12083h	Lifescience Market	96 Tests	EUR 824

Guinea pig Autoimmune Regulator ELISA kit
E05A0527-192T	BlueGene	192 tests	EUR 1270
Description: A sandwich ELISA for quantitative measurement of Guinea pig Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Autoimmune Regulator ELISA kit
E05A0527-48	BlueGene	1 plate of 48 wells	EUR 520
Description: A sandwich ELISA for quantitative measurement of Guinea pig Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Autoimmune Regulator ELISA kit
E05A0527-96	BlueGene	1 plate of 96 wells	EUR 685
Description: A sandwich ELISA for quantitative measurement of Guinea pig Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Mouse Autoimmune regulator (AIRE)
KTE70434-48T	Abbkine	48T	EUR 332
Description: Quantitative sandwich ELISA for measuring Mouse Autoimmune regulator (AIRE) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Autoimmune regulator (AIRE)
KTE70434-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2115
Description: Quantitative sandwich ELISA for measuring Mouse Autoimmune regulator (AIRE) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Autoimmune regulator (AIRE)
KTE70434-96T	Abbkine	96T	EUR 539
Description: Quantitative sandwich ELISA for measuring Mouse Autoimmune regulator (AIRE) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human AIRE (Autoimmune Regulator)
ELK3194	ELK Biotech	1 plate of 96 wells	EUR 432
Description: A sandwich ELISA kit for detection of Autoimmune Regulator from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human AIRE (Autoimmune Regulator)
E-EL-H0536	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human AIRE (Autoimmune Regulator) in samples from Serum, Plasma, Cell supernatant

Basic Protocol 1: Seeding enteroid fragments onto Transwells for monolayer formation Alternate Protocol: Seeding enteroid fragments for monolayer formation utilizing trituration Basic Protocol 2: Isolation of monocytes and derivation of immune cells from human peripheral blood Basic Protocol 3: Isolation of neutrophils from human peripheral blood Basic Protocol 4: Assembly of enteroid/macrophage or enteroid/neutrophil co-culture.