Feasibility, Long-term Safety and Immune Monitoring of Regulatory T Cell Therapy in Living Donor Kidney Transplant Recipients

Feasibility, Long-term Safety and Immune Monitoring of Regulatory T Cell Therapy in Living Donor Kidney Transplant Recipients

Short-term outcomes in kidney transplantation are marred by progressive transplant failure and mortality secondary to immunosuppression toxicity. Immune modulation with autologous polyclonal regulatory T cell (Treg) remedy might facilitate immunosuppression discount selling higher long-term scientific outcomes.

In a Phase I scientific trial, 12 kidney transplant recipients acquired 1-10×106 Treg per kg at Day+5 put up transplantation in lieu of induction immunosuppression (Treg Therapy cohort). Nineteen sufferers acquired customary immunosuppression (Reference cohort). Primary outcomes have been rejection-free and affected person survival. Patient and Transplant survival was 100%; acute rejection-free survival was 100% in the Treg Therapy vs 78.9% in the reference cohort at 48 months post-transplant.

Treg remedy revealed no extra security issues. Four sufferers in the Treg Therapy cohort had mycophenolate mofetil withdrawn efficiently and stay on tacrolimus monotherapy. Treg infusion resulted in a long-lasting dose-dependent improve in peripheral blood Tregs along with a rise in marginal zone B cell numbers. We recognized a pre-transplantation immune phenotype suggesting a excessive threat of unsuccessful ex-vivo Treg enlargement.

Autologous Treg remedy is possible, protected and is probably related to a decrease rejection price than customary immunosuppression. Treg remedy might present an thrilling alternative to attenuate immunosuppression remedy and enhance long-term outcomes.

Immune thrombocytopenia (ITP) World Impact Survey (I-WISh): affected person and doctor perceptions of prognosis, indicators and signs, and remedy

Immune thrombocytopenia (ITP) is now well-known to cut back sufferers’ health-related-quality-of-life. However, knowledge describing which indicators and signs sufferers and physicians understand as having the best impression are restricted, as is knowing the total results of ITP therapies. I-WISh (ITP World Impact Survey) was an exploratory, cross-sectional survey designed to determine the multifaceted impression of ITP, and its therapies, on sufferers’ lives.

It centered on perceptions of 1507 sufferers and 472 physicians from 13 international locations concerning diagnostic pathway, frequency and severity of indicators and signs, and remedy use. Twenty-two p.c of sufferers skilled delayed prognosis (attributable to a number of components), 73% of whom felt anxious in consequence.

Feasibility, Long-term Safety and Immune Monitoring of Regulatory T Cell Therapy in Living Donor Kidney Transplant Recipients

Patients rated fatigue among the many most frequent, extreme symptom related to ITP at prognosis (58% most frequent; 73% most extreme), though physicians assigned it decrease precedence (30%). Fatigue was one of the few signs persisting at survey completion (50% and 65%, respectively) and was the highest symptom sufferers wished resolved (46%). Participating physicians have been skilled at treating ITP, thereby recognizing the necessity to restrict corticosteroid use to newly-diagnosed or first-relapse sufferers and espoused elevated use of thrombopoietin receptor agonists and anti-CD20 after relapse in sufferers with persistent/continual illness.

Patient and physicians have been largely aligned on prognosis, signs, and remedy use. I-WISh demonstrated that sufferers and physicians largely align on total ITP symptom burden, with sure variations eg fatigue. Understanding the emotional and scientific toll of ITP on the affected person will facilitate shared decision-management, setting and institution of remedy targets and illness stage-appropriate remedy choice.

Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells

Human intestinal enteroids derived from grownup stem cells supply a related ex vivo system to review organic processes of the human intestine. They recreate mobile and practical options of the intestinal epithelium of the small gut (enteroids) or colon (colonoids) albeit restricted by the dearth of related cell sorts that assist keep tissue homeostasis and reply to exterior challenges.

In the intestine, innate immune cells work together with the epithelium, assist barrier operate, and deploy effector features. We have established a co-culture system of enteroid/colonoid monolayers and underlying macrophages and polymorphonuclear neutrophils to recapitulate the mobile framework of the human intestinal epithelial area of interest. Enteroids are generated from biopsies or resected tissue from any phase of the human intestine and maintained in long-term cultures as three-dimensional buildings by means of supplementation of stem cell progress components.

Immune cells are remoted from contemporary human complete blood or frozen peripheral blood mononuclear cells (PBMC). Monocytes from PBMC are differentiated into macrophages by cytokine stimulation previous to co-culture. The strategies are divided into the 2 primary parts of the mannequin: (1) producing enteroid/colonoid monolayers and isolating immune cells and (2) meeting of enteroid/colonoid-immune cell co-cultures with separate apical and basolateral compartments. Co-cultures containing macrophages could be maintained for 48 hr whereas these involving neutrophils, resulting from their shorter life span, stay viable for Four hr.

Enteroid-immune co-cultures allow a number of final result measures, together with transepithelial resistance, manufacturing of cytokines/chemokines, phenotypic evaluation of immune cells, tissue immunofluorescence imaging, protein or mRNA expression, antigen or microbe uptake, and different mobile features. © 2020 Wiley Periodicals LLC.

Autoimmune regulator (AIRE) Antibody

48129-100ul 100ul
EUR 399.6

Autoimmune regulator (AIRE) Antibody

48129-50ul 50ul
EUR 286.8

Autoimmune regulator (AIRE) Antibody

48149 100ul
EUR 429

Autoimmune regulator (AIRE) Antibody

48149-100ul 100ul
EUR 399.6

Autoimmune regulator (AIRE) Antibody

48149-50ul 50ul
EUR 286.8

Autoimmune regulator(Aire)Antibody

E80908-2a 100μl
EUR 225
Description: Available in various conjugation types.

Autoimmune regulator (AIRE) Antibody

E81107-1 100ul
EUR 225
Description: Available in various conjugation types.

Autoimmune Regulator (AIRE) Antibody

20-abx325675
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  • 100 ug
  • 50 ug

Autoimmune Regulator (AIRE) Antibody

20-abx312575
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Autoimmune Regulator (AIRE) Antibody

20-abx323992
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  • 100 ug
  • 50 ug

Autoimmune Regulator (AIRE) Antibody

abx331461-100ul 100 ul
EUR 510

Autoimmune Regulator (AIRE) Antibody

abx038141-100ug 100 ug
EUR 469.2

Autoimmune Regulator (AIRE) Antibody

20-abx175527
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  • 1 mg
  • 200 ug

Autoimmune Regulator (AIRE) Antibody

20-abx175528
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  • 1 mg
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Autoimmune Regulator (AIRE) Antibody

20-abx175529
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  • 1 mg
  • 200 ug

Autoimmune Regulator (AIRE) Antibody

20-abx171376
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  • 1 mg
  • 200 ug

Autoimmune Regulator (AIRE) Antibody

abx230241-100ug 100 ug
EUR 577.2

Autoimmune Regulator (AIRE) Antibody

abx117155-100ug 100 ug
EUR 560.4

Autoimmune Regulator (AIRE) Antibody

20-abx007582
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Autoimmune Regulator (AIRE) Antibody

20-abx001466
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Autoimmune Regulator (AIRE) Antibody

20-abx013298
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Autoimmune Regulator (AIRE) Antibody

abx019018-100ug 100 ug
EUR 410.4

Autoimmune Regulator (AIRE) Antibody

abx013298-100g 100 µg
EUR 237.5

Autoimmune Regulator (AIRE) Antibody

abx013298-10g 10 µg
EUR 43.75

Autoimmune Regulator (AIRE) Antibody

abx013298-200g 200 µg
EUR 312.5

Autoimmune Regulator (AIRE) Antibody

abx001466-100l 100 µl
EUR 400

Autoimmune Regulator (AIRE) Antibody

abx001466-20l 20 µl
EUR 175

Autoimmune Regulator (AIRE) Antibody

abx001466-50l 50 µl
EUR 275

Autoimmune Regulator (AIRE) Antibody

abx230241-100g 100 µg
EUR 350

Autoimmune Regulator (AIRE) Antibody

abx038141-1mg 1 mg
EUR 337.5

Autoimmune Regulator (AIRE) Antibody

abx117155-100g 100 µg
EUR 450

Autoimmune Regulator (AIRE) Antibody

abx019018-1mg 1 mg
EUR 300

Autoimmune Regulator (AIRE) Antibody

abx312575-100g 100 µg
EUR 362.5

Autoimmune Regulator (AIRE) Antibody

abx312575-20g 20 µg
EUR 162.5

Autoimmune Regulator (AIRE) Antibody

abx312575-50g 50 µg
EUR 250

Autoimmune Regulator (AIRE) Antibody

abx325675-100g 100 µg
EUR 250

Autoimmune Regulator (AIRE) Antibody

abx325675-50g 50 µg
EUR 187.5

Autoimmune Regulator (AIRE) Antibody

abx323992-100g 100 µg
EUR 250

Autoimmune Regulator (AIRE) Antibody

abx323992-50g 50 µg
EUR 187.5

Rat Autoimmune Regulator ELISA kit

E02A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Autoimmune Regulator ELISA kit

E02A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Autoimmune Regulator ELISA kit

E02A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Autoimmune Regulator ELISA kit

E01A8993 96T
EUR 700
Description: ELISA

Pig Autoimmune Regulator ELISA kit

E07A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Autoimmune Regulator ELISA kit

E07A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Autoimmune Regulator ELISA kit

E07A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Autoimmune Regulator ELISA kit

E08A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Autoimmune Regulator ELISA kit

E08A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Autoimmune Regulator ELISA kit

E08A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Autoimmune Regulator ELISA kit

E01A43914 96T
EUR 700
Description: ELISA

Goat Autoimmune Regulator ELISA kit

E06A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Autoimmune Regulator ELISA kit

E06A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Autoimmune Regulator ELISA kit

E06A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Autoimmune Regulator ELISA kit

E01A0230 96T
EUR 700
Description: ELISA

Human Autoimmune Regulator ELISA kit

E01A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Autoimmune Regulator ELISA kit

E01A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Autoimmune Regulator ELISA kit

E01A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Sheep Autoimmune Regulator ELISA kit

E01A96207 96T
EUR 700
Description: ELISA

Mouse Autoimmune Regulator ELISA kit

E03A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Autoimmune Regulator ELISA kit

E03A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Autoimmune Regulator ELISA kit

E03A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Autoimmune regulator ELISA Kit

EK3200 96 tests
EUR 636

Mouse Autoimmune Regulator ELISA kit

E01A17735 96T
EUR 700
Description: ELISA

Mouse Autoimmune Regulator ELISA Kit

IMSAIREKT each
EUR 752
Description: Mouse Autoimmune Regulator ELISA Kit

Human Autoimmune Regulator ELISA Kit

IHUAIREKT each
EUR 733
Description: Human Autoimmune Regulator ELISA Kit

Canine Autoimmune Regulator ELISA kit

E01A61349 96T
EUR 700
Description: ELISA

Rabbit Autoimmune Regulator ELISA kit

E01A26482 96T
EUR 700
Description: ELISA

Rabbit Autoimmune Regulator ELISA kit

E04A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Autoimmune Regulator ELISA kit

E04A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Autoimmune Regulator ELISA kit

E04A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Bovine Autoimmune Regulator ELISA kit

E01A78778 96T
EUR 700
Description: ELISA

Monkey Autoimmune Regulator ELISA kit

E01A70061 96T
EUR 700
Description: ELISA

Monkey Autoimmune Regulator ELISA kit

E09A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Autoimmune Regulator ELISA kit

E09A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Autoimmune Regulator ELISA kit

E09A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Porcine Autoimmune Regulator ELISA kit

E01A52631 96T
EUR 700
Description: ELISA

Chicken Autoimmune Regulator ELISA kit

E01A87504 96T
EUR 700
Description: ELISA

Autoimmune Regulator (AIRE) Antibody (HRP)

20-abx312576
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  • 100 ug
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Autoimmune Regulator (AIRE) Antibody (HRP)

abx312576-100g 100 µg
EUR 362.5

Autoimmune Regulator (AIRE) Antibody (HRP)

abx312576-20g 20 µg
EUR 162.5

Autoimmune Regulator (AIRE) Antibody (HRP)

abx312576-50g 50 µg
EUR 250

Human Autoimmune Regulator (AIRE) Protein

20-abx652629
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  • 100 ug
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  • 1 mg
  • 200 ug
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Mouse Autoimmune Regulator (AIRE) Protein

20-abx652630
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  • 100 ug
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  • 1 mg
  • 200 ug
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Mouse Autoimmune Regulator (AIRE) Protein

20-abx652631
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  • 100 ug
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  • 1 mg
  • 200 ug
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Autoimmune Regulator (AIRE) Antibody (FITC)

20-abx312577
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  • 100 ug
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Autoimmune Regulator (AIRE) Antibody (FITC)

abx312577-100g 100 µg
EUR 362.5

Autoimmune Regulator (AIRE) Antibody (FITC)

abx312577-20g 20 µg
EUR 162.5

Autoimmune Regulator (AIRE) Antibody (FITC)

abx312577-50g 50 µg
EUR 250

Human Autoimmune Regulator (AIRE) Protein

abx652629-100g 100 µg Ask for price

Human Autoimmune Regulator (AIRE) Protein

abx652629-10g 10 µg
EUR 1800

Human Autoimmune Regulator (AIRE) Protein

abx652629-50g 50 µg
EUR 4525

Mouse Autoimmune Regulator (AIRE) Protein

abx652630-100g 100 µg Ask for price

Mouse Autoimmune Regulator (AIRE) Protein

abx652630-10g 10 µg
EUR 1800

Mouse Autoimmune Regulator (AIRE) Protein

abx652630-50g 50 µg
EUR 4525

Pig Autoimmune Regulator (AIRE) ELISA Kit

abx360670-96tests 96 tests
EUR 990

Rat Autoimmune regulator(AIRE)ELISA kit

YLA1691RA-48T 48T Ask for price

Rat Autoimmune regulator(AIRE)ELISA kit

YLA1691RA-96T 96T Ask for price

Autoimmune Regulator (AIRE) Antibody (Biotin)

20-abx312578
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  • 100 ug
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Autoimmune Regulator (AIRE) Antibody (Biotin)

abx312578-100g 100 µg
EUR 362.5

Autoimmune Regulator (AIRE) Antibody (Biotin)

abx312578-20g 20 µg
EUR 162.5

Autoimmune Regulator (AIRE) Antibody (Biotin)

abx312578-50g 50 µg
EUR 250

Human Autoimmune Regulator (AIRE) CLIA Kit

20-abx493615
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Autoimmune Regulator (AIRE) CLIA Kit

20-abx493616
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Autoimmune Regulator (AIRE) CLIA Kit

abx196471-96tests 96 tests
EUR 990

Human Autoimmune Regulator (AIRE) CLIA Kit

abx196471-100l 100 µl Ask for price

Human Autoimmune Regulator (AIRE) CLIA Kit

abx196471-50l 50 µl
EUR 618.75

Human AIRE -Autoimmune Regulator- CLIA Kit

E-CL-H0428-24Tests 24 Tests
EUR 180
Description: Sandwich

Human AIRE -Autoimmune Regulator- CLIA Kit

E-CL-H0428-48Tests 48 Tests
EUR 546
Description: Sandwich

Human AIRE -Autoimmune Regulator- CLIA Kit

E-CL-H0428-96Tests 96 Tests
EUR 682
Description: Sandwich

Human AIRE -Autoimmune Regulator- CLIA Kit

E-CL-H0428-96Tests10 96 Tests *10
EUR 6820
Description: Sandwich

Human AIRE -Autoimmune Regulator- CLIA Kit

E-CL-H0428-96Tests5 96 Tests *5
EUR 3410
Description: Sandwich

Autoimmune Regulator (AIRE) Cell ELISA Kit

abx595022-100g 100 µg Ask for price

Autoimmune Regulator (AIRE) Cell ELISA Kit

abx595022-10g 10 µg
EUR 493.75

Autoimmune Regulator (AIRE) Cell ELISA Kit

abx595022-50g 50 µg Ask for price

Human Autoimmune Regulator (AIRE) ELISA Kit

DLR-AIRE-Hu 96T
EUR 463
Description: tissue homogenates or other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit

DLR-AIRE-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit

DLR-AIRE-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit

DLR-AIRE-Mu 96T
EUR 475
Description: tissue homogenates or other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit

DLR-AIRE-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit

DLR-AIRE-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Autoimmune Regulator (AIRE) in samples from tissue homogenates or other biological fluids.

Human AIRE/ Autoimmune regulator ELISA Kit

E0100Hu 1 Kit
EUR 515
Description: APECED,Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein,APECED protein

Guinea Pig Autoimmune Regulator ELISA kit

E01A35196 96T
EUR 700
Description: ELISA

Human AIRE(Autoimmune Regulator) ELISA Kit

ELK3194-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AIRE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AIRE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AIRE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AIRE in the samples is then determined by comparing the OD of the samples to the standard curve.

Human AIRE(Autoimmune Regulator) ELISA Kit

ELK3194-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AIRE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AIRE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AIRE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AIRE in the samples is then determined by comparing the OD of the samples to the standard curve.

Guinea pig Autoimmune Regulator ELISA kit

E05A0527-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Autoimmune Regulator ELISA kit

E05A0527-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Autoimmune Regulator ELISA kit

E05A0527-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Autoimmune Regulator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse AIRE(Autoimmune Regulator) ELISA Kit

ELK6262-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AIRE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AIRE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AIRE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AIRE in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse AIRE(Autoimmune Regulator) ELISA Kit

ELK6262-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AIRE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AIRE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AIRE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AIRE in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse Autoimmune regulator (AIRE) ELISA Kit

EK7318 96Т
EUR 799

Human Autoimmune Regulator (AIRE) ELISA Kit

EKN43760-48T 48T
EUR 378.7

Human Autoimmune Regulator (AIRE) ELISA Kit

EKN43760-5x96T 5x96T
EUR 2569.75

Human Autoimmune Regulator (AIRE) ELISA Kit

EKN43760-96T 96T
EUR 541

Mouse Autoimmune Regulator (AIRE) ELISA Kit

EKN43761-48T 48T
EUR 389.62

Mouse Autoimmune Regulator (AIRE) ELISA Kit

EKN43761-5x96T 5x96T
EUR 2643.85

Mouse Autoimmune Regulator (AIRE) ELISA Kit

EKN43761-96T 96T
EUR 556.6

Mouse Autoimmune regulator, Aire ELISA KIT

ELI-11972m 96 Tests
EUR 1038

Human Autoimmune regulator, AIRE ELISA KIT

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EUR 988.8

Human Autoimmune Regulator (AIRE) ELISA Kit

EKU02649-48T 48T
EUR 555.66

Human Autoimmune Regulator (AIRE) ELISA Kit

EKU02649-5x96T 5x96T
EUR 3770.55

Human Autoimmune Regulator (AIRE) ELISA Kit

EKU02649-96T 96T
EUR 793.8

Mouse Autoimmune Regulator (AIRE) ELISA Kit

EKU02650-48T 48T
EUR 571.55

Mouse Autoimmune Regulator (AIRE) ELISA Kit

EKU02650-5x96T 5x96T
EUR 3878.38

Mouse Autoimmune Regulator (AIRE) ELISA Kit

EKU02650-96T 96T
EUR 816.5

Human AIRE (Autoimmune Regulator) ELISA Kit

EKE60371-5x96T 5x96T
EUR 3124.08

Human AIRE (Autoimmune Regulator) ELISA Kit

EKE60371-96T 96T
EUR 657.7

Human Autoimmune Regulator (AIRE) ELISA Kit

DL-AIRE-Hu 96T
EUR 441
Description: tissue homogenates or other biological fluids.

Mouse Autoimmune Regulator (AIRE) ELISA Kit

DL-AIRE-Mu 96T
EUR 453
Description: tissue homogenates or other biological fluids.

Human AIRE(Autoimmune regulator) ELISA Kit

EH1501 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human Autoimmune Regulator (AIRE) ELISA Kit

abx570732-96tests 96 tests
EUR 848.4

Mouse Autoimmune Regulator (AIRE) ELISA Kit

abx570926-96tests 96 tests
EUR 886.8

Human Autoimmune regulator (AIRE) ELISA Kit

AE19265HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)

Human Autoimmune regulator (AIRE) ELISA Kit

AE19265HU-96Tests 96 Tests
EUR 610
Description: Human (Homo sapiens)

Mouse Autoimmune regulator (AIRE) ELISA Kit

AE19266MO-48Tests 48 Tests
EUR 325
Description: Mouse (Mus musculus)

Mouse Autoimmune regulator (AIRE) ELISA Kit

AE19266MO-96Tests 96 Tests
EUR 610
Description: Mouse (Mus musculus)

Human Autoimmune Regulator (AIRE) ELISA Kit

abx250784-96tests 96 tests
EUR 848.4

Mouse Autoimmune Regulator (AIRE) ELISA Kit

20-abx153694
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Autoimmune Regulator (AIRE) ELISA Kit

20-abx150773
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Autoimmune regulator (AIRE) ELISA Kit

KTE60636-48T 48T
EUR 339
Description: This Human Autoimmune regulator (AIRE) ELISA Kit employs a two-site sandwich ELISA to quantitate AIRE.

Human Autoimmune regulator (AIRE) ELISA Kit

KTE60636-96T 96T
EUR 589
Description: This Human Autoimmune regulator (AIRE) ELISA Kit employs a two-site sandwich ELISA to quantitate AIRE.

Human Autoimmune regulator (AIRE) ELISA Kit

KTE60636-96T5 96 T×5
EUR 2629
Description: This Human Autoimmune regulator (AIRE) ELISA Kit employs a two-site sandwich ELISA to quantitate AIRE.

Human Autoimmune regulator (AIRE) ELISA Kit

KTE60636-96T50 96 T×50
EUR 24569
Description: This Human Autoimmune regulator (AIRE) ELISA Kit employs a two-site sandwich ELISA to quantitate AIRE.

Human Autoimmune Regulator (AIRE) ELISA Kit

RDR-AIRE-Hu-48T 48T
EUR 475.34
Description: tissue homogenates and other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit

RDR-AIRE-Hu-48Tests 48 Tests
EUR 652.8

Human Autoimmune Regulator (AIRE) ELISA Kit

RDR-AIRE-Hu-96T 96T
EUR 679.04
Description: tissue homogenates and other biological fluids.

Human Autoimmune Regulator (AIRE) ELISA Kit

RDR-AIRE-Hu-96Tests 96 Tests
EUR 907.2

Basic Protocol 1: Seeding enteroid fragments onto Transwells for monolayer formation Alternate Protocol: Seeding enteroid fragments for monolayer formation utilizing trituration Basic Protocol 2: Isolation of monocytes and derivation of immune cells from human peripheral blood Basic Protocol 3: Isolation of neutrophils from human peripheral blood Basic Protocol 4: Assembly of enteroid/macrophage or enteroid/neutrophil co-culture.

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